A new PCR-based seroneutralization assay in cell culture for diagnosis of hepatitis E

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چکیده

منابع مشابه

A new PCR-based seroneutralization assay in cell culture for diagnosis of hepatitis E.

A new method for the serological diagnosis of hepatitis E virus (HEV) infection based on neutralization of the virus in cell culture was developed. The test involves a short incubation of the virus in the presence of the serum sample to be tested and permissive cells. With viral replication being limited and without a cytopathic effect, viral growth in cells is evaluated by reverse transcriptio...

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A real time PCR assay on blood for diagnosis of invasive candidiasis in immunocompromised patient

Background and Purpose: Invasive candidiasis (IC) is a significant cause of morbidity and mortality in patients with hematologic disorders and bone marrow transplant recipients. Rapid, specific and sensitive test for the timely accuracy in immunocompromised patients to reduce mortality rates and prevent IC progress is necessary. We established a real-time PCR assay on blood for the diagnosis an...

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Comparison of PCR and Culture Methods for Diagnosis of Enteropathogenic Campylobacter in Fowl Feces

Enteritis due to Campylobacter is the most common cause of acute bacterial diarrhea worldwide. In most cases, infection occurs as a result of consuming contaminated water or food, especially raw meat of fowls. Campylobacters are saccharolytic and fastidious bacteria. These traits limit the number of available biochemical tests by which isolates may be differentiated. These limitations might, in...

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Effects of different culture media on optimization of primary neuronal cell culture for in vitro models assay

Background: In vitro model studies are becoming increasingly popular for experimental research designs. They include isolation and expansion of cells of a particular tissue, such as the nervous tissue which contributes to understanding the underlying mechanisms in many pathologies. It enables  the scrutinization of intracellular signaling pathways responsible for cell death. OBJECTIVES: In the ...

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comparison of a pcr-based method with culture and direct examination for diagnosis of acanthamoeba keratitis

background: the aim was to compare three different methods (direct examination, culture and pcr meth­ods) for the diagnosis of acanthamoeba keratitis (ak) in corneal scrapes. methods: twenty eight corneal scrapes and contact lenses were collected from keratitis patients and re­ferred to the de­partment of medical parasitology and mycology, school of public health, tehran univer­sity of medical ...

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ژورنال

عنوان ژورنال: Journal of Clinical Microbiology

سال: 1997

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.35.6.1373-1377.1997